Ammomum subulatum mainly contain the glycosides, petunidin 3, 5-diglucoside, leucocyanidin-3-O-β-D-glucopyranoside, chalcone, cardamonin, flavanone, alpinetin and subulin. Acid hydrolysis of subulin gave the aglycone, subulaurone. The seeds on steam distillation yield a dark brown, mobile essential oil (2.5%) having a characteristic odor of cineol. Volatile oils present in seed containing cineol (74%), limonene (10.3%), myrcene (0.3%), α-terpinene (0.2% and 4-terpinene (0.2%).The seeds of A. subulatum contains the glycosides, Petunidin-3,5-diglucoside, Leucocyanidin-3-O-ß-D glucopyranoside.Subulin (New aurone glycoside) and 1-8, Cineole, α-terpinyl Acetate.