BunnyPure Host Removal Kit

Company Information

BunnyTeeth Technology Inc. is committed to building a complete core technology platform for bioengineering, building a high-quality biotech product chain and a technical service chain, with the ambition to become a global leader in molecular and genetic diagnostic. BunnyTeeth Technology Inc. will also provide customers with a full-range of one-step service solution in the field of molecular diagnostics and genetic diagnostic.

Product Information

BunnyTeeth Technology Inc. uses a unique unbiased lysis technology that can specifically remove host cells and nucleic acids while retaining intact bacteria, fungi, DNA viruses, chlamydia, mycoplasma and other pathogenic microorganisms in the sample. The enriched microorganisms can be used for various downstream applications such as PCR, qPCR, mNGS after DNA/RNA extraction.

Product Advantages

Various Sample Types: Widely support blood, saliva, alveolar lavage fluid, sputum, swabs and other biological fluid samples.

Unbiased: Unbiased microbial nucleic acid extraction, compatible with multiple pathogen types, minimizes microbial loss in samples.

High Efficiency and Simplicity: Fast and simple operation to minimize the host background and enrich microorganisms efficiently.

Wide Applications: Compatible with a variety of downstream applications, including PCR, qPCR, mNGS, etc., ensuring the accuracy and precision of downstream experiments.

Experimental Data

High quality of Microbial DNA Enrichment

Figure 1. Efficient recovery of microbial DNA enrichment.

Use BunnyPure Host Removal Kit and Zymo similar product to remove host DNA and then extract microbial DNA from 5 body fluid samples. Compared to no host removal, BunnyPure Host Removal Kit was highly efficient at removing host DNA, and the extraction yield of BunnyPure kit was consistent with the performance of those of competing products.

Figure 2. Efficient host removal.

Use BunnyPure Host Removal Kit and Zymo similar product to remove host DNA and then extract microbial DNA from 5 body fluid samples. qPCR was performed on the conserved sequences of host DNA. higher Ct values indicated lower host DNA yield. The host removal ability of BunnyPure Host Removal Kit was is the same as similar products from competing companies.

Stable, balanced and accurate sequence information

Figure 3. Excellent QC data for mNGS.

Use BunnyPure Host Removal Kit to remove host DNA in alveolar lavage samples. Microbial DNA obtained with BunnyMag Body Fluids Microbiome DNA Isolation Kit entered the mNGS process with excellent quality control performance.

High Sequencing Abundance and Unbiased Detection of Microbial Fractions

Figure 4. Unbiased DNA extraction for different types of microbiomes.

Z microbial standards were used as test samples into 5 alveolar lavage samples. Host DNA was removed by BunnyPure Host Removal Kit and Zymo similar product and microbial DNA was extracted. Also, library preparation and sequencing were completed. Compared with no host removal, this product was able to efficiently remove host DNA and significantly improve the detection of microorganisms.

Main Composition

Storage Condition

Nuclease is stored at -20℃, Proteinase K at 2-8℃, and other reagents at room temperature.

The validity period is 12 months.

Operating Process