REAGEN™Ciprofloxacin ELISA Test Kit provides a competitive enzyme mmunoassay for the quantitative analysis of Ciprofloxacin in feed, meat,tissue, milk, serum and urine.
The unique features of the kit are:
Rapid, high recovery (75 - 95%), and cost-effective extraction methods for various samples. High sensitivity (0.35 ng/g or ppb) and low detection limit (0.525 ng/g or ppb) for feed samples. High reproducibility. A quick ELISA assay (less than 1.5 hour regardless of number of samples).
The method is based on a competitive colorimetric ELISA assay. The ciprofloxacin antibody has been coated in the plate wells. During the analysis, sample is added along with the ciprofloxacin-horseradish peroxidase (Ciprofloxacin-HRP) conjugate. If the ciprofloxacin residue is present in the sample, it will compete for the ciprofloxacin antibody, thereby preventing the Ciprofloxacin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the ciprofloxacin residue concentration in the sample.
REAGEN™ Ciprofloxacin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.
|
Kit Contents |
Amount |
Storage |
|
Ciprofloxacin-coated Plate |
96-well plate (8 wells x 12 strips) |
2-8°C |
|
Ciprofloxacin Standards: Negative control (white cap tube) 0.35 ng/mL (yellow cap tube) 1.0ng/mL (orange cap tube) 2.5ng/mL (pink cap tube) 7.5ng/mL (purple cap tube) 22.5 ng/mL (blue cap tube) 100ng/mL (spiking, optional, red cap tube) |
1.0mL 1.0mL 1.0mL 1.0mL 1.0mL 1.0mL 1.0mL |
2-8°C |
|
Ciprofloxacin-HRP |
15mL |
2-8°C |
|
10X Sample Extraction Buffer |
25mL |
|
|
20X Wash Solution |
30mL |
|
|
TMB Substrate |
12mL |
|
|
Stop Buffer |
15mL |
|
|
Enro/Cipro Extraction Buffer |
10mL |
|
|
Sample Balance Buffer |
1.6mL |
If you are not planning to use the kit for over 3 months, store Ciprofloxacin standards, Ciprofloxacin-HRP at -20°C or in a freezer.
|
Sample Type |
Detection Limit (ng/g or ppb)(Organic Reagent Extraction Method) |
Detection Limit (ng/g or ppb)(Rapid Method) |
|
Feed |
0.525 |
3.5 |
|
Meat/Liver/Kidney/Fish/Shrimp |
0.525 |
3.5 |
|
Milk |
0.7 |
1.75 |
|
Serum |
0.7 |
1.75 |
|
Urine |
0.7 |
1.75 |
|
Analytes |
Cross-Reactivity (%) |
|
Ciprofloxacin |
100 |
|
Danofloxacin |
33 |
|
Enoxacin |
15 |
|
Pipemidic acid |
13 |
|
Ofloxacin |
8 |
|
Benofloxacin |
3 |
|
Oxolin acid |
4 |
|
Nalidixic acid |
2 |
