Model : DR13-R10k / DR13-R01M / DR12-R10k / DR18-B50h / SOR51-E20h / SOR51-E10k
DiaStarTM RTase is a thermostable mutant of M-MULV reverse transcriptase (RNaseH-) which is a recombinant type revealed and purified from E.Coli, since this is more thermostable than M-MLV, the synthesis of cDNA is possible over 50°C and this shows the optimal activity at 50°C. In addition, the synthesis of cDNA is achieved efficiently because 2nd structure of RNA can be retarded more easily comparing to that in low temperature reaction. (below 42°C)
Application
High-specific Long PCR High G + C and / or complex second structure DNA Amplification of low-copy DNA General sequencing TA cloning Difficult PCR