, is a kind of amino polycarboxylic acid and calcium chelating agent. As an anti-coagulation tube additive, it can effectively prevent blood samples from coagulating. It is suitable for general hematology test. It can also complex some ions in plasma to make some protein or nucleic acid substances more stable.
As an additive of vacuum blood tube, EDTA dipotassium can protect the cell composition of blood, not affect the count and size of white blood cell, has the least effect on the morphology of red blood cell, and can inhibit the aggregation of platelet, and is suitable for general hematology test. In addition, EDTA dipotassium can be used as a chelating agent in toilet deodorants and cosmetics, as a complexing agent in liquid phase analysis, and as a metal polishing agent.
EDTA dipotassium has no effect on white blood cell count and size, and little effect on erythrocyte morphology.
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EDTA dipotassium is usually added into the vacuum blood collection tube for blood routine examination (Note: EDTA dipotassiumis not applicable to the coagulation test and platelet kinetic energy test, nor to the determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase and PCR test). In addition, EDTA dipotassium is generally used as the blood anticoagulant in the nucleic acid detection tube. EDTA dipotassium can also be used for complexing metal ions and separating metals, as well as for detergents, liquid soaps, shampoos, agricultural chemical sprays, antidotes, etc.
|
No. |
Project |
Specifications |
|
1 |
Content (%)≥ |
99.0 |
|
2 |
PH (50g/L,25°C) |
4.8±1 |
|
3 |
Clarity test |
Qualified |
|
4 |
Solubility (%)≥ |
60 |
|
5 |
Chloride (cl)≤ |
0.005 |
|
6 |
Sulfate (SO4)≤ |
0.02 |
|
7 |
Iron (Fe)≤ |
0.001 |
|
8 |
Heavy metal (calculated as Pb)≤ |
0.001 |
|
Note:”%” in the table refers to mass fraction. |
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In the whole blood sample collection test, the key step of preparation is that the blood should be fully contacted with EDTA dipotassium to achieve full anticoagulation. Therefore, it is necessary to gently reverse 5~8 times as soon as possible after blood collection to prevent the formation of tiny blood clots.
Anticoagulation requires a sufficient amount of EDTA, but excessive EDTA may cause morphological changes in blood cells, and accurate control of EDTA dipotassium addition is particularly important.
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