FGF-1 (acidic FGF) is a member of the fibroblast growth factor (FGF) family, which binds heparin. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion (ref.).

Applications

Use as a supplement in serum-free or reduced serum media for culture of mammalian cellsStudies of the human FGF-1 receptor, transmembrane signaling and protein phosphorylationWestern blotting control for anti-EGF-1 antibodiesWidely used in cosmetics, such as whitening, anti-wrinkle, anti-aging, etc.

Specification

Product: Full length recombinant mature FGF-1 (15.8 kDa, 140 amino acids) expressed in E. coli.

Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit (Cell Titer 96,

Promega) with Balb/c 3T3 cells was < 10 pg/ml, corresponding to a specific activity of < 1 x 108 units/mg.

Purity: >98% as determined by SDS-PAGE (CBB staining)

Form: 2.0 mg/ml in PBS (10mM Na-phosphate, 150mM NaCl) pH7.2, 50% glycerol, filter-sterilized

Storage: -20°C or for long-term storage, -70°C