GENEDIA COVID-19 Real-Time RT-PCR kit

GENEDIA W COVID-19 Real-Time RT PCR kit reagent is a test to qualitatively extract SARS-CoV-2 virus RNA from substances of human respiratory organs (Nasopharyngeal, Oropharyngeal swab, Sputum, Bronchoalveolar lavage) on a real-time basis.

SPECIFIC / RELIABLE / VERSATILE

• Target 4 genes including RdRP, N, E, S gene with one tube

• With UDG, increase accuracy and minimize invalid results

• Results available within 100 mins with simple PCR steps

Performance data

Positive: 22, Negative: 20, Total: 42

* PPA (Positive percent agreement): 100%

* NPA (Negative percent agreement): 100%

* OPA (Overall percent agreement): 100%

TEST PROCEDURE

1. Sample extraction

Use conventional RNA Extraction kit

2. Amplification

1) Take out the Reagents, thaw them at room temperature

2) Prepare 20㎕RT-PCR reaction mixture as follows

3) After closing caps, mix thoroughly by tapping the tubes 2~3 times, and then spin down.

3. Data Analysis

SPECIFICATION

Method: Real Time RT-PCR

Packing unit: 100 Tests / kit

Specimen: Nasopharyngeal, Oropharngeal swab, Sputum, Bronchoalveolar lavage

Running time: 100mins

Instrument: ABI 7500/7500 fast(Applied Biosystems),CFX96(Bio-Rad)

Expiry date: 6 months from the date of manufacture

Storage condition: -25 ~ -15℃

Certificates

CE-IVD, ISO, GMP (MFDS of Korea)