<span>It is mainly suitable for the cultivation of various cancer cell lines, some primary cell cultures, and a variety of cell lines,that are relatively easy to feed.</span>

<span>Bovine serum is the most used natural medium for cell culture. It is rich in nutrients necessary for cell growth. It is often used</span><br><span>for in vitro culture of animal cells. It has extremely important functions: (1) Provides hormones for maintaining exponential cell</span><br><span>growth. There are no or very few nutrients in the medium, as well as the main low-molecular nutrients. (2) Provide binding</span><br><span>proteins, which can recognize vitamins, lipids, metals and other hormones, and can bind or modulate the vitality of the substances</span><br><span>they bind. (3) In some cases, the binding protein can combine with toxic metals and pyrogens to detoxify. (4) It is a source of</span><br><span>factors needed for cells to adhere to and spread on the plastic culture medium. (5) Act as a pH buffer. (6) Provide protease</span><br><span>inhibitors to inactivate the remaining trypsin during cell passage and protect cells from harm.</span><br><br><span>According to the bovine birth time and the method of serum collection and separation, serum is generally divided into fetal bovine</span><br><span>serum, newborn bovine serum and calf serum. Fetal bovine serum is made by puncturing the heart of eight-month-old fetal bovine</span><br><span>blood, then heat inactivating, dialysis, and filtration sterilization. This product is a standard fetal bovine serum, which has</span><br><span>been heat-inactivated and filtered to sterilize. It does not need to be heat-inactivated before use. It can be used after slow</span><br><span>thawing at low temperature.</span><br><br><span>Storage and transportation</span><br><br><span>Transported on dry ice; stored at -20°C, valid for 5 years.</span><br><br><span>Instructions for use</span><br><br><span>1. Thawing: A gentle heating process can effectively reduce the generation of precipitation. It is not recommended to thaw serum</span><br><span>directly at high temperature.</span><br><br><span>1) Move the frozen serum to a refrigerator at 2-8°C and thaw overnight;</span><br><br><span>2) Or move the serum to a 37°C water bath, shake and mix the dissolved substance at the bottom intermittently until all is</span><br><span>dissolved.</span><br><br><span>2. The concentration of fetal bovine serum in the basic medium is generally 5-20%, and it is added in proportion to the cell types</span><br><span>and experimental needs.</span><br><br><span>Precautions</span><br><br><span>1. Avoid repeated freezing and thawing. After the serum is thawed, it can be stored at 2-8°C for 6 weeks. If the storage time of</span><br><span>the thawed serum needs to be longer than 6 weeks, it is recommended to divide it into a suitable volume and re-freeze it.</span><br><br><span>2. If the serum contains a large amount of precipitate, it is not recommended to filter the serum directly. If there is a need for</span><br><span>filtration, the serum can be aliquoted into sterile centrifuge tubes, centrifuged slightly, and the supernatant is directly added</span><br><span>to the culture medium and mixed before filtering.</span><br><br><span>3. If the serum is not mixed and placed in a water bath higher than 40°C, the protein at the bottom will be denatured to form a</span><br><span>precipitate.</span>