Product Description
ONLY FOR SCIENCE RESEARCH
Drug Names
Generic Name：Human anti-2019 nCoV(N) IgG ELISA Kit(Qualitative)
Principle
This kit was based on indirect enzyme-linked immune-sorbent assay technology. Recombinant Nucleocapsid protein was pre-coated onto 96-well plates. And the HRP conjugated antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
Sequence of spike protein RBD(antigen)
MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
Materials provided with the kit
|
Materials provided with the kit |
96 determinations |
Storage | |
|
1 |
User manual |
1 |
R.T. |
|
2 |
Closure plate membrane |
2 |
R.T. |
|
3 |
Sealed bags |
1 |
R.T. |
|
4 |
Microelisa stripplate |
1 |
2-8°C |
|
5 |
Standard |
0.5ml×1 bottle |
2-8°C |
|
6 |
Standard diluent |
1.5ml×1 bottle |
2-8°C |
|
7 |
HRP-Conjugate reagent |
6ml×1 bottle |
2-8°C |
|
8 |
Sample diluent |
6ml×1 bottle |
2-8°C |
|
9 |
Chromogen Solution A |
6ml×1 bottle |
2-8°C |
|
10 |
Chromogen Solution B |
6ml×1 bottle |
2-8°C |
|
11 |
Stop Solution |
6ml×1 bottle |
2-8°C |
|
12 |
wash solution |
