Synonyms:4-Aminobutyric Acid

Product Name：Human GABA(Gamma-aminobutyric acid) ELISA Kit

Catalog NO.：FY-EH4566

Reactivity：General

Assay Length：3h

Detection Range：4.94–400pg/mL

Detection Method：Competitive Inhibition

Size：48T, 96T

Storage：2–8 ℃ for 6 months

Sensitivity：＜2.17pg/mL

CV(%)：Intra-Assay: CV<10%； Inter-Assay: CV<12%

Note：For Research Use Only

GABA(Gamma-aminobutyric acid) Basic information

Gamma-Aminobutyric acid, or γ-aminobutyric acid, or GABA , is the chief inhibitory neurotransmitter in the developmentally mature mammalian central nervous system. Its principal role is reducing neuronal excitability throughout the nervous system.

GABA is sold as a dietary supplement in many countries. It has been traditionally thought that exogenous GABA (i.e. taken as a supplement) doesn’t cross the blood-brain barrier, however data obtained from more current research indicates that it may be possible.

Human GABA(Gamma-aminobutyric acid) ELISA Kit Test method

This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

Reference:

1.”Blood–brain barrier to H3-γ-aminobutyric acid in normal and amino oxyacetic acid-treated animals”.

2. “Neurotransmitters as food supplements: the effects of GABA on brain and behavior”

3. “A point mutation in a Drosophila GABA receptor confers insecticide resistance”.