Human IL-6(Interleukin 6) ELISA Kit Basic Information
Product Name | Human IL-6(Interleukin 6) ELISA Kit |
Catalog NO. | FY-EH3991 |
Alias | IL-6 ELISA Kit, Interleukin 6 ELISA Kit, IL6 ELISA Kit, BSF2 ELISA Kit, HGF ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, CDF ELISA Kit |
Application | IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in serum, plasma, tissue homogenates and other biological fluids. |
Size | 48T, 96T |
Storage | 2-8 ℃ for 6 months |
Sensitivity | <2.813pg/ml |
Species | Human |
Uniprot | P05231 |
CV (%) | Intra-Assay: CV<8% |
Note | For Research Use Only |
Human IL-6(Interleukin 6) Introduciton
Interleukin-6, abbreviated as interleukin-6, was originally discovered by the team of Japanese immunologist Chuzo Kishimoto. It is an interleukin that mediates inflammation (but in some cases it also has an anti-inflammatory effect). Interleukin-6 can be secreted by T cells, B cells, monocytes, fibroblasts, keratinocytes, endothelial cells, mesangial cells, and some tumor cells. Interleukin-6 was originally thought to be a factor that can promote the differentiation of B cells into plasma cells, but other functions have been discovered later. For example, interleukin-6 can cross the blood-brain barrier, which is related to the normal physiological process of the central nervous system and the process of damage repair.
Human IL-6(Interleukin 6) ELISA Kit Test method
This kit uses sandwich ELISA to detect the concentration of Human IL-6.
Human IL-6-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human IL-6 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human IL-6 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.