Intended use

This ELISA kit is intended for use in quantitating Carboxypeptidase B, recombinant from Rattus norvegicus. The kit is for research use only and is not intended for diagnostic use in human or animals.

Principle of the Procedure

This Carboxypeptidase B assay is a two-site immuno enzymetric assay. The recombinant Carboxypeptidase B from Rattus norvegicus, expressed in E.Coli, from YAXINBIO, in the samples are reacted in micro titer wells coated with a purified capture antibody. An anti-Carboxypeptidase B monoclonal antibody conjugated with the enzyme horseradish peroxidase (HRP) is reacted simultaneously forming a sandwich complex of solid phase antibody-Carboxypeptidase B-HRP labeled antibody. After a wash step to remove any unbound reactants, the wells are then reacted with tetramethylbenzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of recombinant Carboxypeptidase B present in the samples. Accurate quantitation is achieved by comparing the signal of recombinant Carboxypeptidase B standards assayed at the same time.