Ordinary culturemediaUses: promote the growth ofmicroorganisms; accumulate metabolites; isolate microbialspecies; identify microbial species; count microbial cells;preserve microbial species; prepare microbialproducts.
General configuration steps of ordinaryculture medium
2. dissolution
3. Adjust PH
Filter paper or cotton.(sometimes it can be omitted)
6. Packing
Carry out high-pressure steamsterilization according to the temperature and pressure requiredon the formula. If the sterilization temperature is too high, thenutrients will be destroyed and cultured. The sugar and aminoacid in the ordinary culture medium will darken the color of themedium.
9. 1. Choose the rightnutrients
When the concentration ofnutrients in the medium is appropriate, the growth ofmicroorganisms can be good. When the concentration of nutrientsis too low, it can not meet the needs of normal growth ofmicroorganisms. When the concentration is too high, it mayinhibit the growth of microorganisms.
4. redoxpotential
In the preparation of culturemedium, we should try to use cheap and easy material to obtainraw materials as the components of culture medium, especially inthe fermentation industry, and the amount of culture medium isvery large, and the use of low-cost raw materials reflects itseconomic value.
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