Proteinase K is a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations. It degrades proteins even in the presence of detergents. Proteinase K cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

PROPERTIES

Molecular weight： 29 kDa (SDS-PAGE)

Isoelectric point： 7.81

Optimum pH： 7.0-12.0

Optimum： 65 ℃

APPLICATIONS

Genetic diagnostic kit; RNA and DNA extraction kits; Extraction of non-protein components from tissues, degradation of protein impurities, such as DNA vaccines and preparation of heparin; Preparation of chromosome DNA by pulsed electrophoresis; Western blot; Enzymatic glycosylated albumin reagents in vitro diagnosis.

Superiority

1. Higher stability and enzyme activity based on directed evolution technologies

2. Tolerant of Guanidine salt

3. Free of enzyme impurities and heavy metal ions, DNA <5 pg/mg

4. Annual output of 1500 kg, the largest proteinase K supplier in China