Proteinase K
Protease K is a serine protease with efficient enzymatic activity and broad substrate specificity, preferentially breaking down ester and peptide bonds adjacent to the C-terminus of hydrophobic, sulfur-containing, and aromatic amino acids, and is often used to degrade proteins to produce short peptides. It has the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases and has two Ca2+ binding sites around the active center to increase its stability, allowing it to maintain high enzymatic activity under a wider range of conditions.
Technical Features
| Appearance | Colorless to light brown liquid |
| Specific activity | ≥800 U/mL |
| Concentration | ≥20 mg/mL |
| Deoxyribonuclease activity | Deoxyribonuclease activity not detected |
| Ribonuclease activity | Ribonuclease activity not detected |
Attributes
|
Source |
Tritirachium album |
Remark |
|
Classification |
EC 3.4.21.64 |
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|
Molecular weight |
29 kDa (SDS-PAGE) |
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|
Isoelectric point |
7.81 |
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Optimum pH |
7.0-12.0 all have high activity |
Fig. 1 |
|
Temperature |
65 ℃ |
Fig. 2 |
|
pH Stability |
pH 4.5-12.5 (25 ℃, 16 h) |
Fig. 3 |
|
Thermal stability |
Stable below 50 ℃ （pH 8.0, 30min） |
Fig. 4 |
|
Activators |
SDS, Urea |
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|
Inhibitors |
Di-isopropyl fluorophosphate (DFIP), benzene sulfonyl fluoride (PMSF) |
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|
Storage conditions |
2-25 ℃ in 12 months |
|
Application
1. Genetic diagnostic kits;
2. RNA and DNA extraction kits;
3. Extraction of non-protein components from tissues, degradation of protein-like impurities, such as DNA vaccine and heparin preparation;
4. Preparation of chromosomal DNA for pulse electrophoresis;
5. Protein blotting;
6. Development and mass production of enzymatic glycated albumin reagents for IVD reagents.
Precaution
Wear protective gloves and goggles and keep well ventilated. The product may cause allergic skin reactions.
