Product description

Optimized for extracting DNA from seed and leaf samples, which have rigid cell walls and are difficult to perform lysis, by using specially formulated polymers. The lysis step is simple as only a single type of column is required. High concentration, high yield of plant DNA is extracted.

Feature

1) Efficient extraction using specially formulated polymers

2) User-friendly spin column method

3) Simple lysis protocol, shredder column not used which improves gDNA yield

4) No organic solvent precipitation step

5) Excellent quality, extensive validation performed against various seeds and leaves

6) QC performed 2 times to minimize lot-to-lot variation

7) Instant response to customer inquiries or complaints

Specification

Product name : PURETM Strong Plant gDNA Extraction Kit

Package : 100 tests/kit

Storage condition : Can be stored at room temperature up to 1 year. Proteinase K and RNase A may be stored at room temperature before initial use. After dissolution with distilled water, recommended to aliquot and store at –20°C in order to avoid repeated freezing and thawing.

Sample types that can be used : Plant seed and leaves

MOQ : No MOQ required

Certificates of manufacturing facility : ISO9001, ISO14001, ISO13485

Lysis flow chart

※ When polymers are mixed with samples and vortexing is performed, the polymers physically interact and disrupt plant cell walls of seed and leaves very efficiently.

QC data

Thorough quality control

Q&A

1. Q : What are the features of the PURE™ series that make it different from other extraction products?

A : Other products use pretreatment(shredder) columns and require two types of spin columns, resulting in a long or complicated extraction process. In addition, some products use organic solvents such as phenol and chloroform. The PURE™ series developed by Infusion Tech does not use pretreatment columns and enables high-purity, high-concentration nucleic acid purification with a single spin column. The precipitation process using organic solvents is also not required, making it a quick and easy three-step process (lysis, wash, elute).

2. Q : What is the difference between your PURE™ Plant gDNA Extraction Kit and the PURE™ Strong Plant gDNA Extraction Kit?

A: The PURE™ Strong Plant gDNA Extraction Kit has a specially formulated polymer added to the standard product, enhancing extraction efficiency from leaves and seeds that are difficult to break down. The lysis efficiency is greatly increased because the polymer can attach to the cell due to the charge difference and disrupts cell walls. You may try out PURE™ Strong Plant gDNA Extraction Kit not only for samples that are difficult to extract but also samples that you have been using previously.

3. Q : How do I use SDL1 buffer?

A : Since SDL1 buffer is a specially formulated polymer and has the properties of an insoluble powder, it is natural for precipitates to form. We recommend using the buffer immediately after vortexing, before precipitation occurs.

4. Q : Is it possible to perform follow-up experiments (NGS, Sequencing, PCR) with the PURE™ Strong Plant gDNA Extraction Kit?

A : PURE™ Strong Plant gDNA Extraction Kit has the advantage of extracting high purity and high concentration of nucleic acids, so it is possible to perform subsequent experiments. Extracted gDNA can be used in various experiments including NGS, sequencing, PCR, electroporation, and cloning.

5. Q : Are the components of the PURE™ Strong Plant gDNA Extraction Kit sterile?

A : The components of the PURE™ Strong Plant gDNA Extraction Kit are sterile.

6. Q : Which buffers in the PURE™ Strong Plant gDNA Extraction Kit do I add ethanol to?

A : SDB buffer and SDW buffer should be used after adding ethanol. If you want to check for other components, please refer to the MSDS of the product.

7. Q : How is product quality control (QC) performed for the PURE™ Strong Plant gDNA Extraction Kit?

A : The QC of all products is performed by randomly selecting products of the same LOT after production and performing extraction using our standard sample. The test methods are quantification, electrophoresis, and PCR. Products are released if they meet our criteria.

8. Q : How do I use lyophilized components (Proteinase K, RNase A)?

A : The lyophilized components should be used after adding the appropriate amount of distilled water specified in the manual and completely dissolving the powder. After initial dissolution and use, store at –20℃. It is recommended to store in aliquots as performance may decrease if they go through repeated freezing and thawing.

9. Q : The filter was clogged while using it, why?

A : The amount of sample used to perform extraction is presumed to be high. We recommend re-experimenting within the sample volume range specified in the manual. If the amount of sample is small, it is likely that a low concentration will be measured when quantitating, so please reduce the amount of elution buffer.

10. Q : There are white powder on the wall of the spin column. Is there something wrong with the product?

A : The powder on the wall of the spin column is filter powder caused by static electricity during the manufacturing process. When the solution pass through the filter, the powder will be caught by the filter, and will not influence purity of the eluted DNA.

Introduction of manufacturer

Infusion Tech was founded on 2020. We have focused on developing nucleic acid preparation and molecular diagnosis PCR kits.

We have over 20 patents on novel nucleic acid extraction methods which we have integrated into our lysis buffers of some of our products.

We also have universal extraction kits using silica membrane technology.We have plenty experience performing extraction against various types of research and IVD use samples. Through our combined experience we were able to release high-quality research purpose kits.

Our biggest confidence is in our products quality. We perform extensive quality control, we make sure lot-to-lot variation is minimized.

Many users in Korea have recognized the stable quality of our kits and are changing from market leader kits to ours.

We exhibited our products at many prestigious international medical exhibitions including AACC, MEDICA and MEDLAB.

We welcome you to experience the high quality of our kits.

Manufacturing facility certificates

Manufacturer facilities

International fair exhibition