Recombinant Carboxypeptidase B
Catalog Number:RCPB01
CAS: 9025-24-5
EC: 3.4.17.2
Storage temperature: 2-8℃
Source: Recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B
1.DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.
2.MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | White or off white, or yellowish powder |
| Specific activity | ≥170 units/mg pro. |
| Purity(SDS-PAGE) | Single major band |
| Molecular Weight(SDS-PAGE) | 33.8±3.4 kDa |
UNIT DEFINITION：One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.
3.RECOMMEND USAGE
Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃.
It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
①Animal origin free: Recombinant carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.
②Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
③High purity: Higher specific activity.No other contaminating proteases such as chymotrypsin and carboxypeptidase A.Host protein residues is less than the limits of biological products.
④Lyophilized powder: The product is lyophilized powder and is convenient to store and transport.
⑤Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
⑥Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6.PRODUCT USAGE
The production of recombinant insulin and its analogues;
the quality test of recombinant antibodies;
the determination of C-terminal amino acids of proteins;
the production of other recombinant polypeptides;
and the enzymatic synthesis of certain specific compounds.
7.RELATED PRODUCTS
Recombinant trypsin
Sequencing grade trypsin
Recombinant human trypsin
Recombinant enterokinase
