The Swab/Saliva Viral DNA/RNA Extraction Kit (Spin Column) is suitable for rapid extraction of high purity viral nucleic acid from plasma, serum, nasopharyngeal swab, sputum, bronchus/alveolar lavage fluid, ascites, culture cell supernatant and urine.

This kit is based on the silica gel column purification method.

The sample is homogenized in the lysis buffer and the nucleic acid is released into the buffer.

The lysis buffer contains a high concentration of guanidine. In this condition, the membrane absorbs nucleic acid by hydrogen bond and electrostatic physical and chemical action, while proteins and other impurities are not absorbed.

The lysate is transferred to the adsorption column for filtration, and the nucleic acid filter membrane is washed to remove residual proteins and other impurities, and finally eluted by the low-salt buffer solution.

The obtained nucleic acids can be directly used in downstream related experiments such as reverse transcription, PCR, RT-PCR, fluorescence quantitative PCR, second-generation sequencing and Northern hybridization.